2nd Virtual Symposium on Urinary EVs
Registration is open until February 14th, 2023 and is free for junior researchers.
Welcome to the 2nd FISEV Seminar
February 6th, 2023, at 9.00 – 17.00 EET
University of Eastern Finland, Snellmania, Seminar room sn210
Yliopistonranta 1, 70210 Kuopio.
Hybrid event will be free of charge to all FISEV Members.
The second FISEV seminar organized by the Finnish Society of Extracellular Vesicles is open to all interested in extracellular vesicle research and it offers excellent possibility for Finnish research teams to report they research and network. We encourage all to join either at place in Kuopio or virtually through TEAMS.
We have the honor to welcome Professor Susanne Gabrielsson from Karolinska Institute as a Keynote Speaker. In addition, leading Finnish scientists in EV field will share their visions on timely topics while our sponsor companies will share their latest advances in product development.
If you want to contribute to FISEV seminar as presenter, please send the 2500-character abstract at the time of registration through the link below. The deadline for registration and abstract submission is 25.1.2023.
Registration for the seminar through the following link https://link.webropolsurveys.com/S/90BA5160D4916372
Seminar price for non FISEV members 35 €. FISEV members are free of charge.
To confirm your registration, please make the payment with following information:
IBAN FI60 5780 3820 1851 62, BIC OKOYFIHH. Include the message text to the payment “FISEV2023, NAME(S) OF REGISTERING PERSON(S)”.
The number of on-site participants is limited to 50. Rest of the interested can participate via Teams. The link will be sent by 5.2.2023 by email.
STATUTORY ANNUAL MEETING will be held in TEAMS February 5th, 2023, 18.00 EET. Link to the TEAMS meeting and agenda will be sent to all members by email.
2nd FISEV Seminar – program
Place: University of Eastern Finland, Kuopio campus, Snellmania building, room Sn210
9:00-9:10 Opening words by the President of FISEV Saara Laitinen
CATEGORIZING EVs (Chair: Tarja Malm)
9:10-9:30 Welcome lecture: “Correlative light and electron microscopy in EV research” by Kirsi Rilla
9:30-10:30 Keynote lecture: “Extracellular vesicles for cancer immunotherapy” by Professor Susanne Gabrielsson, Karolinska Institutet
10.30-10.45 Finnadvance (sponsor talk): “A microfluidic platform to assess the transport and delivery of extracellular vesicles” by Sebastien Mosser
WHEN NOT ONLY CONTENT, BUT ALSO THE FUNCTIONS MATTER (Chair: Kirsi Rilla)
11:00-11:30 “Why we need to develop functional EV assays: case platelet” by Pia Siljander
11:30-11:45 “Estrogen deﬁciency caused by menopause blunts acute exercise-induced circulating microRNA response in extracellular vesicles” by Sira Karvinen
11:45-12.00 “Adipocytes alleviate obesity-induced inﬂammation and metabolic disturbance via increased secretion of extracellular vesicles” by Johanna Matilainen
12:00-12:15 “Extracellular vesicles derived from proinﬂammatory macrophages induce inﬂammatory and invasive responses in melanoma cells” by Kaisa Mäki-Mantila
12:15-12:30 ONI (sponsor talk): “Profiling of EV populations and single EVs with the Nanoimager, a benchtop super-resolution microscope” by Lara Laparra
12:30-13:15 Lunch break (at canteen at own cost)
FISHING OUT VESICLES FOR BIOMARKER PURPOSES (Chair: Sanna Pasonen-Seppänen)
13:15-13:45 “Extracellular vesicles and liquid biopsy sampling on anti-cancer therapy response evaluation” by Arto Mannermaa
13:45-14:00 “Validation of surface protein and capture tools for downstream analysis of astrocyte derived EVs” by Genevieve Bart
14:00-14:15 “Identifying glucose deprivation associated EV based miRNA changes in MCF7 breast cancer cells and their subsequent role in cancer pathogenesis” by Saket Ahuja
14:15-14:30 “Advanced fluorescence microscopies for drug delivery studies” by Elina Vuorimaa-Laukkanen
14:30-14:45 NanoFCM (sponsor talk): “NanoAnalyzer: Combining flow cytometry & particle analysis to meet the challenges of the nano-scale” by Alice Law
EV BASED THERAPIES (Chair: Sanna Pasonen-Seppänen)
15:00-15:30 ”The Great Balls on Fire – EV Sparkle of Life, Disease and Therapies” by Seppo Vainio
15:30-15:45 “CD44 mediates interactions of hyaluronan-coated extracellular vesicles with target cells” by Heikki Kyykallio
15:45-16:00 “Platelet and red blood cell concentrate derived extracellular vesicles targeting into different immune cells” by Petra Ilvonen
16:00-16:15 “Time-gated Raman spectroscopy and proteomics analyses of hypoxic and normoxic renal carcinoma extracellular vesicles” by Anatoliy Samoylenko
16:15-16:30 Luminex (sponsor talk): “Amnis CCD Based Flow Cytometry for High-Sensitivity EV Analysis” by Owen Hughes
16:30-16:45 Wrapping up the meeting
FINDOS Winter Symposium 2022 on Oral Mucosal Diseases and Extracellular Vesicles, 24-25 November 2022
On-site at Meilahti campus of the University of Helsinki/ virtual attendance also possible.
Register at https://forms.office.com/r/DffZyAYN1u.
Detailed program can be viewed below.
METVES II Workshop on the standardization of extracellular vesicle concentration measurements. 24 November, 11.30 AM – 5.30 PM Finnish time. Register at http://www.metves.eu/registration to receive link for virtual meeting.
Keystone Conference on Extracellular vesicles, October 30th – November 2nd 2022, Santa Fe, New Mexico, USA. Abstract submissions and registration are still open! The event program can be found below.
Blood EVs: An ISEV Workshop
1st – 2nd September 2022, with Educational Day on 31st August
“Blood EVs” is an ISEV Workshop on all things related to extracellular vesicles of blood: how to obtain, profile, and use them. The event will be held in Helsinki, Finland, from 1-2 September 2022 and will be preceded by an Education Day on 31 August. The Workshop will be in-person only, and participants have already been selected and notified. However, there will be a virtual option for Education Day. If you would like to participate virtually in the Education Day, please register here: https://www.surveymonkey.com/r/BloodEducationDay
This Blood EVs Educational Day is endorsed by the International Society on Thrombosis and Haemostasis.
View the full program below. Short talks and roundtable discussions will revolve around four themes:
Day 1. Blood EVs as biomarkers
- Roundtable 1: Blood as matrix, choosing the sample analytics
- Roundtable 2: Development of diagnostics, towards clinical feasibility
Day 2. Blood EVs as therapeutics
- Roundtable 3: Decades of knowledge in transfusion medicine: benefits and disadvantages of blood EVs
- Roundtable 4: Regulation of novel blood-based nanomedicines
Only ISEV members may attend the Workshop; if you have been invited and are not a member, please visit https://www.isev.org/page/Membership to sign up. A poll on EV practices will be sent out in advance of the meeting.
Workshop Chairs and Organizing Committee
ISEV WORKSHOP – Blood EVs
“Getting blood-derived EVs into the clinics”
Moderator Rienk Nieuwland, Academic Medical Centre Amsterdam & Pia Siljander, University of Helsinki
9:00-9.20 General welcome to the Educational day & Blood EV workshop (Saara Laitinen, Finnish Red Cross Blood Service & Pia Siljander, University of Helsinki)
9:20-9:50 Introduction to blood EVs (Rienk Nieuwland, Academic Medical Centre Amsterdam)
9:50-10:20 Blood as a source of EVs (Edit Buzás, Semmelweis University)
10:20- 10.50 Detection methods for EVs in blood (Ken Witwer, John Hopkins University)
11:20-11:50 Pre-analytical considerations for blood collection and processing in translational and clinical EV research (Fabrice Lucien-Matteoni, Mayo Clinic)
11:50- 12:20 A clinician’s perspective on blood EV biomarkers (Aleksandra Gasecka, Medical University of Warsaw & Academic Medical Centre Amsterdam)
12:20-13:05 Company presentations (Izon, ParticleMetrix, TimeGate Instruments, BioNavis, Carmine Therapeutics)
14.00-14:30 Reference values of blood EVs (Metka Lenassi, University of Ljubljana)
14:30-15:00 Approaching clinical feasibility with EV flow cytometry (Edwin van der Pol, Academic Medical Centre Amsterdam)
15:00- 15:20 Company presentations (Luminex, Sartorius)
15:50-16.20 Heterogeneous platelet-EVs: theranostic opportunities? (Pia Siljander, University of Helsinki)
16:20-16:50 Post-isolation modifications of EVs for therapeutic purposes (Minh Le, National University of Singapore)
16:50-17:20 Summary and discussion (Rienk Nieuwland, Academic Medical Centre Amsterdam)
17:20-18:00 Results of the attendant survey & the blood EV analyses by participating technologies
19.00 Welcome reception at the Botanic gardens
ISEV WORKSHOP ON BLOOD EVs
DAY 1 Program Blood EVs – matrix prerequisites & technologies & biomarkers
Moderators Pia Siljander (University of Helsinki) & Edit Buzás (Semmelweis University) & Rienk Nieuwland (Academic Medical Centre Amsterdam) & Lorraine O’Driscoll (Trinity College)
9:00-9:10 Introduction to working in the workshops, Pia Siljander
9:10-9:25 Introduction to the topic: Blood as a source of EVs Part I (Edit Buzás & Rienk Nieuwland)
9:25 Comparison of collection tubes and processing intervals for extracellular vesicle analysis in blood (An Hendrix, Ghent University)
9:35 Influence of isolation and anticoagulant on analysis of plasma-derived EVs by imaging flow cytometry (Tobias Tertel, University Hospital Essen)
9:45 Characterization of platelet-derived extracellular vesicle subpopulations (Johanna Puutio, University of Helsinki)
9:55 Plasma EV ID: Fluorescence-based phenotyping of blood-borne extracellular vesicles (Danilo Mladenovic, Tallinn University)
5 minutes break
10:25 Label-free detection and characterization of single sub-micrometer particles in plasma of prostate cancer patients (Agustin Enciso-Martinez, Leiden University Medical Center)
10:35 Stratification of Breast cancer risk by Raman Spectroscopy characterization of Extracellular Vesicles (Lorena Signati, University of Milan)
10:45 Optimization of mutational analysis workflow using next-generation sequencing on plasma derived extracellular vesicles (Rebekka van Hoof, Flemish Institute for Technological Research)
11:10-11:15 Break and organization into three roundtables
11:15-12:30 Roundtable discussions (60’+ 15’ wrap-up in writing by the moderators)
Topics will raise organically from the participants/ electronic platform or by the chairs, for example
- What do we need to consider regarding blood EV pre-analytics, isolation, analytics, applications
- What do we not understand yet?
Table 1 Moderators: Rienk Nieuwland/Pia Siljander
Table 2 Moderators: John Nolan/Edwin van der Pol
Table 3 Moderators: An Hendrix/Metka Lenassi
13:30-14:00 Highlights from the morning discussions by roundtable moderators (3 x 5 minutes & audience)
14:00-14:15 Introduction to the topic: Biomarker applications of the blood EV surface Part I
(Lorraine O’Driscoll, Trinity College)
14:10 Blood EV protein corona: the opening of a new biomarker discovery landscape? (Annalisa Radeghieri, University of Brescia/CSGI, Florence)
14:20 Tumour-derived DNA is enriched as short fragments associated with extracellular vesicles (Daniel Hagey, Karolinska Institutet)
14:30 The HLA-I immunopeptidome of blood EV -detection of clinically relevant peptides (Simon Powis, University of St. Andrews)
Biomarker applications of the blood EV surface Part II
15:25 Methods comparison for plasma-derived EVs isolation to perform proteomics analysis (Sandrine Reymond, University of Geneva)
15:35 Identification of B-cell signatures in plasma EVs from pediatric BCP-ALL patients (Marit Inngjerdingen, Oslo University Hospital)
15:45 Circulating extracellular vesicles provide valuable protein biomarkers in metastatic breast cancer (Lorena Martin-Jaular, Institut Curie)
10 minutes break & moving to roundtables
16:10-17:25 Roundtable discussions (60’+ 15’ wrap-up in writing by the moderators)
Topics will raise organically from the participants/ electronic platform or by the chairs
Table 1 Moderators Lorraine O’Driscoll/Natasha Zavroni
Table 2 Moderators Annalisa Radeghieri/Mari Palviainen
Table 3 Moderators Fabrice Lucien-Matteoni/ Lorena Martin-Jaular
5 minutes break & moving to main hall
17:30-18:00 Highlights shared from the afternoon discussions (3 x 5 min & audience)
ISEV WORKSHOP on BLOOD EVs
DAY 2 Program Blood EVs as Therapeutics
Moderators Minh Le & Saara Laitinen
9.00-9.30 Introduction to topics: Blood as a therapeutic EV source and in delivery of EV-therapy
Standing on the shoulders of giants, decades of transfusion & blood cell therapy (Saara Laitinen, Finnish Red Cross Blood Service)
Potential therapeutic applications of blood cell derived EVs (Minh Le, National University of Singapore)
9.30 Using plasma derived EVs as drug delivery vehicles (Maria Chiara Ferri, University of Genova)
9.40 Delivery of therapeutic mRNA via extracellular vesicles to express soluble proteins (Muhammed Nawaz, University of Gothenburg)
9.50 Development of designed targeting peptides for EV (Sujaan Das, Crane Biosciences)
10-10:15 questions & discussion
10.15 Developing drug delivery RBCEVs produced from preloaded RBC (Diana Piedrahita, Erytech Pharma)
10.25 Characterising safety and efficacy of RBC-EV as therapeutic delivery vehicles for retinal degenerations (Rakshanya Sekar, Australia National University)
10.35 Using RBC-EVs as drug delivery particles (Don Haut, Carmine Therapeutics)
10:45- 11:00 questions
11.00-11.15 Break and organization into three roundtables
Suggestions for roundtable discussion topics in addition to those which are raised organically
- Standing on the shoulders of giants, decades of transfusion knowledge
- Why and what we should consider?
- Benefits and disadvantages of using RBC-EVs as therapeutics
- Technologies for loading and targeting of RBC EVs
- Blood group and tissue type: challenge or opportunity?
- The effect of blood components on delivering EV
- Blood – friendly highway or hostile environment?
- The effect of processing and storage on EVs
11.15-13.00 Roundtable discussions
Roundtable 1 Red blood cell -derived nanobiotechnology
Moderators Minh Le & Kai Härkönen
Roundtable 2 Different blood cell derived EVs for therapy development
Moderators Saara Laitinen & Ulla Impola
Roundtable 3 Lessons learned from blood EVs: is there advantage?
Moderators Edith Buzás & Pia Siljander
LUNCH 13.00 – 14.00
14.00-15.00 Wrap-up the Roundtables (Big hall)
15.30-17:00 Summary of the Workshop Days 1 & 2
Moderators: Saara Laitinen & Pia Siljander & Rienk Nieuwland
Wrap up of the three days into deliverables
- Impact and considerations of technological choices on blood EV isolation, detection and analyses (data from blood EVs and sponsor analytics, survey, roundtable outcomes)
- Therapeutic use of blood cell -derived EVs
What should we have discussed and what next?
Welcome to the 1st FISEV Seminar
15th February 2022 at 9.00 – 16.00 EET
Online event will be free of charge to all FISEV Members
This 1st seminar organized by Finnish Society of Extracellular Vesicles is open to all interested in extracellular vesicle research and it offers excellent possibility for Finnish research teams and companies to network. We encourage all to join this newly formed network of nanobiotechnology to accelerate Finnish contribution to this field. Seminar consists of three sessions: EV imaging, EVs in pathophysiology and the development of EV based therapies. Leading Finnish scientists in EV field will share their visions on these timely topics while our sponsor companies will share their latest advances in product development.
If you want to contribute to FISEV seminar as presenter, please send the 2500 character abstract of your presentation to email@example.com. The deadline for abstracts is 31.1.2022.
Registration for the seminar through the link. Registration deadline 10.2.2022.
Seminar price for non FISEV members 35 €.
FISEV members are free of charge.
To confirm your registration, please make the payment with following information: IBAN FI60 5780 3820 1851 62, BIC OKOYFIHH. Include the message text to the payment “FISEV2022, PAID PERSONS”.
Due to the Covid-19 pandemic the seminar will be held in TEAMS and the participant number is limited to 200.
The 1st FISEV seminar programme
9.00 Opening words
Pia Siljander, University of Helsinki
9.10 Introducing the Finnish Society of Extracellular Vesicles
Saara Laitinen, Finnish Red Cross Blood Service
9.30 KEYNOTE: Engineering extracellular vesicles for targeted drug delivery
Samir El Andaloussi, Karolinska Institutet, Sweden
SESSION 1: Extracellular vesicles in physiology and pathophysiology
Chair Tarja Malm, University of Eastern Finland
10.15 Extracellular vesicles in physiology and neuropathology
Tarja Malm, University of Eastern Finland
10.30 Extracellular vesicles in ischemic stroke
Nea Korvenlaita, University of Eastern Finland
10.45 Sponsor session: NanoFCM
10.55 Transcriptome of brain-dead donor plasma extracellular vesicles – A new perspective on the physiological consequences after brain death
SeoJeong Joo, University of Helsinki
11.10 Sponsor session: HansaBioMed Life Sciences
SESSION 2: Extracellular vesicle imaging
Chair Kirsi Rilla, University of Eastern Finland
11.30 Extracellular Vesicles Under the Microscope – Is Seeing Believing?
Kirsi Rilla, University of Eastern Finland
11.50 Time-resolved fluorescence microscopy studies on cellular up-take of extracellular vesicles
Elina Vuorimaa-Laukkanen, Tampere University
12.05 Arctic exposomes flavoring nature derived exosomes
Feby Pratiwi, Oulu University
12.20 LUNCH with a possibility to network in seminar themed breakout rooms
13.00 Sponsor session: ONI
SESSION 3: Extracellular vesicles in therapy development
Chair Seppo Vainio, Oulu University
13.10 Can we learn to harvest the value of the garden of extracellular vesicles?
Seppo Vainio, Oulu University
13.30 A quick pipeline to obtain extracellular vesicles from cancer spheroids using nanocellulose
Heikki Kyykallio, University of Eastern Finland
13.45 Sponsor Session: Finnadvance
13.55 Piecing together the big picture: Novel detection methods for total extracellular vesicle populations in EVE Consortium
Johanna Puutio, University of Helsinki
14.10 Sponsor session: Wyatt
14.30 Ultracentrifugation used in combination with filtration to isolate the small extracellular vesicles from the cell culture supernatant fraction
Ilana Chefetz, University of Minnesota, USA
14.45 Sponsor session: KDBio
14.55 OPEN DISCUSSION: “EVians in Finland“
Sami Valkonen, University of Helsinki & University of Eastern Finland
15.30 FISEV reminder & closing remarks
Saara Laitinen, Finnish Red Cross Blood Service
Pia Siljander, University of Helsinki